Then, the selection of sorbent combination (primary secondary amine (PSA), octadecylsilane (C18), SAX (strong ion exchange), florisil, aluminum oxide) was performed for further clean-up. To optimize the QuEChERS technique, AA was firstly extracted using acetonitrile from aqueous extract with or without n-hexane as the primary cleaning approach. For this purpose, a QuEChERS (quick, easy, cheap, effective, rugged, and safe) protocol combined with HPLC–PDA was established for determination of AA in various roasted date seed varieties including Sayer, Shahani, Mazafati and Kabkab which the last one was used in the optimization experiments. fruit, were roasted and analyzed for its acrylamide (AA) content for the first time. In the present study, date seeds as the main by-product of Phoenix dactylifera L.
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